奶牛精子变形期间Pld6基因表达及蛋白序列分析

doi:10.11843/j.issn.0366-6964.2018.12.012

Abstract

Abstract:

In order to reveal the mechanism of sperm mitochondrial sheath formation and improve the fertility of bulls, testis and cauda epididymidis of 3 health Holstein bulls aged 15-17 months were collected. The round and elongated sperm cells were collected throung frozen section and (laser capture microdissection,LCM) technique. Epididymal spermatozoa were obtained by floating method. TRizol was used to extract sample RNA. The differences between transcriptomes of germ cells during the spermiogenesis of dairy cows has been analysed and verified by qPCR. To understand the function of the Pld6 gene differentially expressed during spermiogenesis, the structure and function of the Pld6 protein were predicted by bioinformatics analysis. The results showed that, during spermiogenesis, the transcription of Pld6 gene increased during the development from round to elongated spermatids, and then decreased or even shut off during sperm maturation. The gene was enriched in the mitochondrial fusion term and associated with distribution and morphological structure of mitochondria on the midpiece of sperm tail. The 10-32th amino acids of Pld6 was a transmembrane region in cattle. The tertiary structure of Pld6 was horn-like with a conserved β-sheet active site H(X)K(X4)D (HKD) inside, which was similar to the mouse Pld6 structure. And there was a closest evolutionary trend of Pld6 between mouse and cattle. Therefore, according to the studies about mouse, it is concluded that, during the process of bovine spermaiogenesis, increasing expression of Pld6 gene induces the cluster of mitochondria in the midpiece of sperm tail, which promotes the formation of normal mitochondrial sheaths and plays an important role in ensuring bovine sperm viability and fertilizing ability.

CLC Number:

S823.9⁺1.2

LI Xiao-jun, LI Xin, CHEN Xiao-li, ZHAO Yi-qiang, LU Yong-qiang, WANG Dong. The Transcription of Pld6 during Spermiogenesis and the Analysis of Its Protein Sequence in Dairy Cows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2632-2640.

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The Transcription of Pld6 during Spermiogenesis and the Analysis of Its Protein Sequence in Dairy Cows

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